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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.507 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.178 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.273 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.211 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.474 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.160.14 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.1.297 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AH.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.1.280 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.597 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.177.45 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.177.72 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.177.43 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.177.85 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.66 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.72 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.133 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.5.1 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.43.7 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.33.1 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| XD | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.493 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.351.2 (Beta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.640.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.1.352 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.1.251 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| HC.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XCJ | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| FT.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| FZ.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| FY.4 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| B.1.497 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| EN.1.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| AY.30 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.85 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| XBB.6.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| BF.21 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| FK.1.3.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XBF | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| BA.5.6 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.11.5 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XBF.7.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -1649.2 | South Korea |
| FY.5.3 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| FP.2.1.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XBB.2.9 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| FL.2.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| JW.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| JL.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| B.1.571 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| AY.55 (Delta) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |