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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
AY.3.4 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AY.3.4 (Delta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.391	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.391	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.393	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.393	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.432	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.432	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.388	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.388	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
P.6	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
P.6	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.48	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.48	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.112	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.112	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.274	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.274	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.515	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.515	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.258.23	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.258.23	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.370	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.370	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.118	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.118	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.137	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.137	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.309	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.309	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.632	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.632	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.72	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.72	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.108	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.108	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.332	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.332	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.42.1 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AY.42.1 (Delta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.348	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.348	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.428.2	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.428.2	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AJ.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AJ.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.459	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.459	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.160.29	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.160.29	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used