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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.184	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.187	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.187	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
D.3	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
D.3	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AE.2	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AE.2	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.110.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.110.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.342	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.342	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.500	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.500	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.511	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.511	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.428.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.428.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.506	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.506	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.482	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.482	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.335	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.335	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.36.36	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.36.36	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-10849.5	Japan
B.1.499.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.499.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.38	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.38	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.116	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.116	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.134	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.134	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.393	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.393	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.381	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.381	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
C.32	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
C.32	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AE.8	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AE.8	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.516	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-1648.07	Singapore
B.1.1.516	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.526	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.526	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.279	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.279	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.51	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.2.51	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.2.3.18	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used