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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
FK.1.2.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
XBC.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
XBC.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BQ.1.26.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BQ.1.26.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
DS.3	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
DS.3	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.5.1.11	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.5.1.11	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
FS.1	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
FS.1	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
XBB.1.5.54	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
XBB.1.5.54	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BF.29	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BF.29	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
BA.5.2.29	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
BA.5.2.29	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
N.5	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
N.5	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
P.1.15 (Gamma)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
P.1.15 (Gamma)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.348	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.348	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.575	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.575	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
C.26	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
C.26	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
Q.4 (Alpha)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
Q.4 (Alpha)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.1.442	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.1.442	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
P.1.1 (Gamma)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
P.1.1 (Gamma)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
A.2.5.2	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
A.2.5.2	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.600	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.600	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
P.1.12 (Gamma)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
P.1.12 (Gamma)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
B.1.525 (Eta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
B.1.525 (Eta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.6 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AY.6 (Delta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.5.5 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AY.5.5 (Delta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
P.1.7 (Gamma)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
P.1.7 (Gamma)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.24 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
AY.24 (Delta)	ORF1ab	NIID WH-1 F509R854	CAGAAGTTGTTATCGACATAGC	22	40.91	837	816	R	-21257.9	Japan
AY.45 (Delta)	N	Young-N	CTCAGTCCAAGATGGTATTTCT	22	40.91	28583	28604	F	-21953.9	Singapore
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used