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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.626 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| B.1.629 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| B.1.629 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21568.6 | Japan |
| GA.9 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| GA.9 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| AY.61 (Delta) | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| AY.61 (Delta) | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BA.2.75.6 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BA.2.75.6 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| GC.2 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| GC.2 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BQ.1.1.16 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BQ.1.1.16 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| B.1.1.524 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| B.1.1.524 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BA.5.2.38 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BA.5.2.38 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BA.1.15.3 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BA.1.15.3 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| B.1.1.15 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| B.1.1.15 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| AY.59 (Delta) | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| AY.59 (Delta) | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| XBR | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| XBR | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| DJ.1.1.1 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| DJ.1.1.1 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| CH.1.1.21 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| CH.1.1.21 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| AZ.5 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| AZ.5 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| B.1.1.193 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| B.1.1.193 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BL.4 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BL.4 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| FP.3 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| FP.3 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| DA.1 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| DA.1 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| AY.68 (Delta) | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| AY.68 (Delta) | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BQ.1.1.14 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BQ.1.1.14 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| BA.1.16.1 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| BA.1.16.1 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| DN.1.1.1 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| DN.1.1.1 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| B.1.160.28 | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| B.1.160.28 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -21257.9 | Japan |
| AY.5.2 (Delta) | N | Young-N | CTCAGTCCAAGATGGTATTTCT | 22 | 40.91 | 28583 | 28604 | F | -21953.9 | Singapore |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |