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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| MB.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| MB.1 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -2556.23 | Japan |
| MB.1 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -5266.28 | Japan |
| LE.1 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| LE.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| LE.1 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -16362.7 | Japan |
| LE.1 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -16797.4 | Japan |
| XBB.1.5 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| XBB.1.5 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| XBB.1.5 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -20966 | Japan |
| XBB.1.5 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -21400.8 | Japan |
| LF.3 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| LF.3 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| LF.3 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -16362.7 | Japan |
| LF.3 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -16797.4 | Japan |
| MD.1.1 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| MD.1.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| MD.1.1 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -16362.7 | Japan |
| MD.1.1 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -16797.4 | Japan |
| LP.3 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| LP.3 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| LP.3 | S | NIID WH-1 F24381R24873 | TCAAGACTCACTTTCTTCCAC | 21 | 42.86 | 24364 | 24384 | F | -16362.7 | Japan |
| LP.3 | S | NIID WH-1 Seq F24383R24865 | AAGACTCACTTTCTTCCACAG | 21 | 42.86 | 24366 | 24386 | F | -16797.4 | Japan |
| JN.1.5 | ORF1ab | CN-CDC-ORF1ab | CCCTGTGGGTTTTACACTTAA | 21 | 42.86 | 13342 | 13362 | F | -20516.2 | China |
| JN.1.5 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| B.1.1.192 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| JN.1.39.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| ME.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| LE.1.3.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| XDR.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| JN.1.49.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| EG.5.1.3 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| HV.1.6 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| XBB.1.16.11 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| FL.15.1.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| GK.1.1.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| JD.1.1.5 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -14403.2 | France |
| B.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| B.1.1.441 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| B.1.509 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| B.1.293 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| B.1.1.225 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| B.1.637 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| AY.39 (Delta) | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| AY.26 (Delta) | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| AY.103 (Delta) | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| AY.3 (Delta) | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| JN.1.18.7 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| MB.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| LE.1 | ORF1ab | Pasteur-ORF1ab-2 | GGTAACTGGTATGATTTCG | 19 | 42.11 | 14080 | 14098 | F | -18581.4 | France |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |