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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.75.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.2.75.3 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XAK | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XAK | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XAA | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XAA | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| BQ.1.30 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BQ.1.30 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| BQ.1.1.50 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BQ.1.1.50 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| BA.5.1.36 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.5.1.36 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XBB.1.23 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.23 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| XBB.1.38.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.38.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| N/A | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| N/A | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XAA | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -19568.4 | US |
| BQ.1.30 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| BQ.1.1.50 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| BA.5.1.36 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| XBB.1.23 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| XBB.1.38.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| N/A | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| AY.42.1 (Delta) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.348 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.428.2 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| AJ.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.459 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.160.29 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.415.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.243.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.1.98 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.566 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.1.480 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.579 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| N.10 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| P.1.11 (Gamma) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.435 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.445 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.124 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.384 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| XAQ | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| BA.2.3.8 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.569 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.524 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| A.23 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.596.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| B.1.473 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -23117.2 | US |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |