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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.582	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.582	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.582	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.580	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.580	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.580	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.306	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.306	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.306	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.280	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.280	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.280	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.1.329	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.1.329	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.1.329	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.396	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.396	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.396	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.595.3	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.595.3	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.595.3	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.2	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.2	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.2	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.311	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.311	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.311	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.369	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.369	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-16167.4	Japan
B.1.369	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.367	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.367	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.367	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.595	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.595	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
B.1.595	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
B.1.240	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
B.1.240	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
XBB.1.5.61	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
XBB.1.5.61	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
XBB.1.5.61	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
FR.1.2	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
FR.1.2	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
FR.1.2	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
FL.2.2.1	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
FL.2.2.1	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-19557.5	Japan
FL.2.2.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-20677.6	Japan
FL.25	N	HKU-N	CGAAGGTGTGACTTCCATG	19	52.63	29254	29236	R	-20936.4	Hong Kong
FL.25	ORF1ab	NIID WH-1 Seq F519R840	GACATAGCGAGTGTATGCC	19	52.63	823	805	R	-20165.2	Japan
FL.25	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used