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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.5 (Omicron) | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.5 (Omicron) | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -17733.1 | Japan |
| BA.5.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.5.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.5.5 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| CH.1.1.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CH.1.1.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| CH.1.1.2 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BE.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BE.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BE.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BR.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BR.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BR.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| CH.1.1.28 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CH.1.1.28 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| CH.1.1.28 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -17733.1 | Japan |
| BF.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BF.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BF.5 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -17733.1 | Japan |
| CH.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CH.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| CH.1.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BF.7.6 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BF.7.6 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BF.7.6 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BA.5.2 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BA.5.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.5.2 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| CH.1.1.19 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| CH.1.1.19 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| CH.1.1.19 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BM.4.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BM.4.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BM.4.1.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BA.5.2.35 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -4270.22 | Thailand |
| BA.5.2.35 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.5.2.35 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BA.4.6.5 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.4.6.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -19212.8 | Germany |
| BA.4.6.5 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BA.2.18 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.18 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.2.18 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| BA.2.12.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| BA.2.12.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| BA.2.12.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| LZ.1.1 | N | NIH-TH_N | CCCCACTGCGTTCTCCATT | 19 | 57.89 | 28376 | 28358 | R | -3370.08 | Thailand |
| LZ.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -23131.5 | Germany |
| LZ.1.1 | ORF1ab | NIID WH-1 F509R854 | CTCGAACTGCACCTCATGG | 19 | 57.89 | 492 | 510 | F | -21969.5 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |