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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.451 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.451 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.451 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.1.8 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.1.8 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.1.8 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.313 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.313 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.313 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.402 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.402 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.402 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.609 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.609 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.609 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.1.344 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.1.344 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.1.344 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.556 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.556 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.556 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.245 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.245 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.245 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.1.403 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.1.403 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.1.403 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.636 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.636 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.636 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| B.1.126 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| B.1.126 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| B.1.126 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| BQ.1.1.47 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| BQ.1.1.47 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| BQ.1.1.47 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -21956.6 | US |
| XDQ.3 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| XDQ.3 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| XDQ.3 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| JD.1.1.2 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| JD.1.1.2 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| JD.1.1.2 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| XBC.1.1 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| XBC.1.1 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| XBC.1.1 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| FY.9 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| FY.9 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| FY.9 | N | US-CDC-N-3 | GGGAGCCTTGAATACACCAAAA | 22 | 45.45 | 28681 | 28702 | F | -22824.1 | US |
| XBB.1.31.2 | E | Charité-E | ATATTGCAGCAGTACGCACACA | 22 | 45.45 | 26381 | 26360 | R | -23527.6 | Germany |
| XBB.1.31.2 | N | CN-CDC-N | CAGACATTTTGCTCTCAAGCTG | 22 | 45.45 | 28979 | 28958 | R | -23140.2 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |