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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBB.1.19.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GE.1.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| EG.6.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -5057.65 | Germany |
| FU.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| DV.7.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| BA.2.86.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.3.12 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GN.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JG.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JD.1.2.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JD.1.1.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JR.1.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GJ.1.2.6 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.30 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XDA.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.58.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XBB.1.5.33 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.13.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GK.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -5057.65 | Germany |
| XDC | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FL.15.4 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JR.1.1.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -5057.65 | Germany |
| JD.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JG.3.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| KE.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HV.1.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| BA.2.86.5 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.8 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.26.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XCP | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FL.1.8 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| EG.6.1.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| EG.5.1.12 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JF.1.1.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.11 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.3.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| XDE | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| KE.3 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HF.1.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| HK.22 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| FW.1.1.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.13 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| KH.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| LM.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.17 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.15 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.1.2 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.63.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| JN.1.27 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| GK.1.6.1 | ORF1ab | Charité-ORF1b | CARATGTTAAASACACTATTAGCATA | 26 | 23.08 | 15530 | 15505 | R | -9191.15 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |