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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| GK.1.5 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GK.1.5 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| JE.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| JE.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| JD.1.1.7 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| JD.1.1.7 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XCK | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XCK | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| GN.1.4 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GN.1.4 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XBB.1.16.27 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.16.27 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| GY.1.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GY.1.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| HH.8 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HH.8 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| HK.25 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HK.25 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| EG.5.1.11 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| EG.5.1.11 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| HK.9 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HK.9 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| XBB.2.3.15 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.2.3.15 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| GK.2.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GK.2.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| HK.1.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HK.1.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| HN.4.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HN.4.2 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| XBB.1.16.17 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.16.17 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| GK.1.10 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GK.1.10 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| FL.13.4.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| FL.13.4.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| EG.5.1.15 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| EG.5.1.15 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| JY.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| JY.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| XBB.1.16.24 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.1.16.24 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| HK.17 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HK.17 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -15630.5 | France |
| DV.7.1.7 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| DV.7.1.7 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| DV.7.1.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| DV.7.1.3 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| JP.1.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| JP.1.1 | ORF1ab | Pasteur-ORF1ab-1 | CTCCCTTTGTTGTGTTGT | 18 | 44.44 | 12797 | 12780 | R | -18837.8 | France |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |