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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.317 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| B.1.1.317 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| B.1.1.317 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| B.1.1.397 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| B.1.1.397 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| B.1.1.397 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| B.1.1.397 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| CM.6.1 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| CM.6.1 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BA.5.2.54 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BA.5.2.54 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BA.5.2.54 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BA.5.2.54 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| DR.2 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| DR.2 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| DR.2 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| DR.2 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BF.7.27 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -13753.9 | France |
| BF.7.27 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BF.7.27 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BF.7.27 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| B.1.1.26 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| B.1.1.26 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| B.1.1.26 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| B.1.1.26 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| CM.8 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| CM.8 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| CM.8 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| CM.8 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BA.5.1.27 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BA.5.1.27 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BA.5.1.27 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BA.5.1.27 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BF.16 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BF.16 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BF.16 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BF.16 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BA.5.5.3 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BA.5.5.3 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BA.5.5.3 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BA.5.5.3 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BA.5.2.59 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BA.5.2.59 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BA.5.2.59 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BA.5.2.59 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BF.15 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| BF.15 | E | Niu-E | TTCTTGCTTTCGTGGTATTC | 20 | 40 | 26303 | 26322 | F | -19913.4 | China |
| BF.15 | E | Niu-E | CACGTTAACAATATTGCAGC | 20 | 40 | 26391 | 26372 | R | -20723.8 | China |
| BF.15 | N | US-CDC-N-2 | TTACAAACATTGGCCGCAAA | 20 | 40 | 29164 | 29183 | F | -21115.5 | US |
| BQ.1.15 | ORF1ab | Pasteur-ORF1ab-2 | CTGGTCAAGGTTAATATAGG | 20 | 40 | 14186 | 14167 | R | -18348.8 | France |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |