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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.489	ORF1ab	Young-ORF1ab	CACTTAATGTAAGGCTTTGTTAAG	24	33.33	14243	14220	R	-22408	Singapore
B.1.489	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-9191.15	Germany
B.1.489	ORF1ab	Won-ORF1ab	TGCATTAACATTGGCCGTGA	20	45	15558	15539	R	-21175.8	South Korea
B.1.489	ORF1ab	Chan-ORF1ab	GTGTGATGTTGAWATGACATGGTC	24	41.67	16353	16330	R	-13317.5	China
B.1.489	ORF1ab	HKU-ORF1b	AACRCGCTTAACAAAGCACTC	21	42.86	18909	18889	R	-17655.9	Hong Kong
B.1.489	S	Young-S	ATCCAGCCTCTTATTATGTTAGAC	24	37.5	21876	21853	R	-22070.7	Singapore
B.1.489	S	Chan-S	CAAGCTATAACGCAGCCTGTA	21	47.62	22869	22849	R	-22073.5	China
B.1.489	S	Won-S	CACCTGTGCCTGTTAAACCA	20	50	23213	23194	R	-21965.9	South Korea
B.1.489	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-19261.4	South Korea
B.1.489	E	Charité-E	ATATTGCAGCAGTACGCACACA	22	45.45	26381	26360	R	-23527.6	Germany
B.1.489	E	Huang-E	GCAGCAGTACGCACACAATC	20	55	26376	26357	R	-23540.5	China
B.1.489	E	Niu-E	CACGTTAACAATATTGCAGC	20	40	26391	26372	R	-20723.8	China
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used