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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.305 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -18242.2 | South Korea |
| BZ.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| BQ.1.1.19 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| KP.2.3.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| BC.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1.1.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1.1.3 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1.1.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| DJ.1.2 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| C.20 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| XAY.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| BA.2.86.7 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| KP.2.3.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| KP.2.3.4 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| KP.2.3.3 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| BA.5.3.5 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| GL.3 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| GL.1 | N | Corman-N | CACATTGGCACCCGCAATC | 19 | 57.89 | 28706 | 28724 | F | -18248.7 | Germany |
| BQ.1.10.2 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -18248.8 | South Korea |
| FL.10.2 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -18248.8 | South Korea |
| BQ.1.2.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| CM.7.1.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.2.86.7 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| EN.1.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.21 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.5.6 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.11.5 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XAM | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.1.30 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.9 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XAC | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.1.1.11 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XZ | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.1.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.4.6.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XBJ | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| DQ.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BF.31.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.1.77 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.1.21.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.5.1.37 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| EE.3 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| ES.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| EE.5 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.1.52 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BA.5.2.32 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| BQ.1.1.58 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| XAP | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |