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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| EG.5.1.8 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JG.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| EG.5.1.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| EG.5.1.6 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| GK.1.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| FL.1.5.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JF.1.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| GK.1.8 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JD.1.1.8 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| HK.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| EG.5.1.14 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JN.1.7.9 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KW.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| MF.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| LF.2 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KR.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JN.1.7.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KP.2.3.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KR.1.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| JN.1.49 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| MF.2 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.5.2.54 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| DR.2 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BF.7.27 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| B.1.1.26 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| CM.8 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.5.1.27 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BF.16 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.5.5.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.5.2.59 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BF.15 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BQ.1.15 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| CA.5 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| CP.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XBB.3.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| ED.3 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| CM.4 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BQ.1.4 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| CA.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BN.1.9 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BQ.1.28 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| DG.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| BA.5.2.56 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KP.2.13 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| XDP.2 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| HN.8 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| KL.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| HK.24.1 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| GK.2.4 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| FL.1.5.2 | S | Chan-S | CCTACTAAATTAAATGATCTCTGCTTTACT | 30 | 30 | 22712 | 22741 | F | -27219.1 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |