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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.77	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.1.149	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.491	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.619.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.1.50	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.398	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
C.8	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
C.27	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.182	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.160.7	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.1.303	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.619	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.282	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.36.7	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.45	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.1.193	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
C.38	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	185.411	Japan
B.1.160.26	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	92.4864	US
B.1.277	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	92.4864	US
B.1.444	N	US-CDC-N-3	GGGAGCCTTGAATACACCAAAA	22	45.45	28681	28702	F	92.4864	US
XBF.7.1	S	Young-S	TATACATGTCTCTGGGACCA	20	45	21763	21782	F	29.6049	Singapore
BA.5.2.20	S	Young-S	TATACATGTCTCTGGGACCA	20	45	21763	21782	F	29.6049	Singapore
BF.5	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
XBB.1.5.60	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.2 (Omicron)	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
XBF.5	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.258.2	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.58	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
XBB.2.3.10	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
FW.2	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
FY.1.2.2	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.277	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.1.412	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
EG.9	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.1.3	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.2.75.10	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BN.1.5.1	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.2.10.4	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
FF.1	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BM.4.1	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
FC.1	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
C.13	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.6	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.1.21.1	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.177.49	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
AY.39.3 (Delta)	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
BA.2.33	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.1.53	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
B.1.258.23	S	Chan-S	CCTACTAAATTAAATGATCTCTGCTTTACT	30	30	22712	22741	F	-51.5687	China
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used