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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
XAV	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-22616.4	Germany
BE.1.4.4	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-22616.4	Germany
BF.20	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-22616.4	Germany
BA.2.77	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-22616.4	Germany
CP.1.3	E	Charité-E	ACAGGTACGTTAATAGTTAATAGCGT	26	34.62	26269	26294	F	-22616.4	Germany
B.1.609	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22621.2	Japan
DN.1.1.4	N	NIH-TH_N	CGTTTGGTGGACCCTCAGAT	20	55	28320	28339	F	-22623	Thailand
XBB.3.2	N	NIH-TH_N	CGTTTGGTGGACCCTCAGAT	20	55	28320	28339	F	-22623	Thailand
BM.4	N	NIH-TH_N	CGTTTGGTGGACCCTCAGAT	20	55	28320	28339	F	-22623	Thailand
B.4.1	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
B.4.7	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
BA.5.10	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
BA.5.10.1	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
FW.1.1	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
AY.50 (Delta)	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
B.1.595.3	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
DF.1.1	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
DF.1	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
XBB.2.11	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
AY.46.2 (Delta)	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
AY.114 (Delta)	ORF1ab	NIID WH-1 F501R913	CTTTACCAGCACGTGCTAGAAGG	23	52.17	896	874	R	-22644.4	Japan
AY.121 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.14 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.119 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.46.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.114 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.54 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.64 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.46.4 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.107 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.81 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.117 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.118 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.27 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.617.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.67 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.110 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.39.1 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.20 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.42 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.62 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.113 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.4 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.43 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.98.1 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.127 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.83 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.4.2 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
AY.1 (Delta)	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
B.1.1.172	E	Won-E	TTCGGAAGAGACAGGTACGTT	21	47.62	26259	26279	F	-22650	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used