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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| C.27 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.421 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.364 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.82 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.61 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.47 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.38 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.251 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| A.2.3 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.288 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.249 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.378 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.97 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.9 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.439 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.408 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.256 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| Q.7 (Alpha) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.460 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| EG.9 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBU | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.351.5 (Beta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FZ.1.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBB.1.9.7 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBC.1.6.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FL.4.4 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FQ.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBB.1.17 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FL.3.4 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FZ.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| DV.7.2 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FL.6 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FL.30.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBB.1.5.68 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| CD.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.122.3 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.512 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.349 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.265 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.401 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.362 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.1.6 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FW.1.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| HN.3.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.1.1.17 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BS.1.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| CM.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.2.75.7 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| CA.7 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.453 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |