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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| EG.9.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| XBB.2.3.22 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| JV.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.36 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BE.1.1.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.23 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.3.9 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1.8 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| DV.6 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2 (Omicron) | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5.5.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.6 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.3 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.75.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1.66 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.3.12 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.13 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.9 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BM.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5.1.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.15 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1.19 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.3.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BQ.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| DV.3 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.4.1.8 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BN.1.3 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5.1.22 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.75 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5 (Omicron) | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.5.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BE.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BR.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.28 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| CH.1.1.19 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BM.4.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.4.6.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.18 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| BA.2.12.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| LZ.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| KP.3.1.5 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| KW.1.1.1 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| KU.2 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -22019.3 | Thailand |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |