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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.76 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.301 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.36.7 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.269 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.234 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| Z.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| V.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.36.22 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.282 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.131 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.274 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| A.25 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.235 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.177.65 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.45 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.165 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.258.12 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBS | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BN.1.2.8 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAH | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.5.2.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBJ.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EP.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.4.1.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| CL.1.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBF.8.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAU | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAY.1.1.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.25.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAD | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAB | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| CH.1.1.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FT.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAL | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBW | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.3.7 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAY.1.1.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.19 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GY.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBF.10 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GA.8 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XAY.1.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.38 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| CT.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.10.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FF.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.1.99 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBF.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| B.1.343 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |