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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| CZ.2 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.5.1.18 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.1.29 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.4.1.2 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.47 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| FK.1.2.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XBC.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.26.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| DS.3 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.5.1.11 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| C.37 (Lambda) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| P.1.14 (Gamma) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| P.1.7.1 (Gamma) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| P.1.2 (Gamma) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.112 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| P.1.10 (Gamma) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| A.2.5 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.102 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.124 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.46.6 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.43.3 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.119.1 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.25.2 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.122.6 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| B.1.621.2 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.39.2 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.125 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.103.1 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.92 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.122.4 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| AY.101 (Delta) | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.1.5 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.1.14 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.57 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.49 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BE.3 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.4.8 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.9.6 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.13 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.69 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XM | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.1.1.4 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.70 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.2.63 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BA.4.1.4 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BQ.1.8.2 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| EC.1.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BM.1.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| XL | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| BD.1 | N | NIID-JP-N | TGGCAGCTGTGTAGGTCAAC | 20 | 55 | 29282 | 29263 | R | -20522.3 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |