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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.356 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.462 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| A.29 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.147 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| P.1.6 (Gamma) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| P.1.5 (Gamma) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| P.1.4 (Gamma) | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.420 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.39.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.233 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.320 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| C.33 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.635 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| C.21 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| C.14 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| N.4 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| AM.2 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.113 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.5.2 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.423 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.36.39 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.57 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| AH.3 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.289 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.365 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.252 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.640.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.5 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.428 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.355 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.52 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.465 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.160.33 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| S.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.221.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.258.3 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.215 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.36.20 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.82 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.462 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.218 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.74 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| AP.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| AK.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.1.13.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.203 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.254 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.36.12 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.21 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| AQ.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |