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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| B.1.1.257 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.5.4 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.323 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.15 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.105 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.4.4 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.398 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.94 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| HK.18 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.18 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.17 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.164 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.275 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.258.2 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.58 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.81 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.419 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.151 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.36.2 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| V.2 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.149 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.413 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.258.4 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.9.5 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.107 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.10 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.9 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.4.10 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.47 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.36.16 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.41 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.71 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AS.1 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.142 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.166 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.4.11 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.4 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.34.2 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.50 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.60 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.52 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| W.3 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.250 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.63 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.27 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.23 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.130 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.1.44 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.469 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| B.1.177.10 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |