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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XAY.1.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BQ.1.1.55 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BY.1.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| EA.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XJ | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BR.5 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBF.8 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.5.1.29 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| CM.5.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| CM.8.1.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBS | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BN.1.2.8 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAH | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.5.2.5 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBJ.3 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| EP.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.4.1.5 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.4 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| CL.1.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBF.8.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAU | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAY.1.1.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.25.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAD | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAB | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| CH.1.1.4 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| FT.4 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAL | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBW | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.3.7 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAY.1.1.3 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.19 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| GY.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBF.10 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| GA.8 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XAY.1.1.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBB.1.38 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| CT.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.10.4 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| FF.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.60 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.9.2 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.11 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.2.3.15 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.5.6.1 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| BA.5.1.35 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| XBB.1.45 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| B.1.177.31 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| B.1.160.26 | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| AY.39.1.2 (Delta) | S | WuhanCoV-spk1 | TGTGGTTCATAAAAATTCCTTTGTG | 25 | 32 | 24900 | 24876 | R | -23857.7 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |