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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.39	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1480.18	South Korea
GN.4	ORF1ab	NIID WH-1 F501R913	TTCGGATGCTCGAACTGCACC	21	57.14	484	504	F	-1474.95	Japan
B.1.36.22	ORF1ab	NIID WH-1 F501R913	TTCGGATGCTCGAACTGCACC	21	57.14	484	504	F	-1474.95	Japan
B.1.1.112	ORF1ab	NIID WH-1 Seq F519R840	ACCTCATGGTCATGTTATGG	20	45	502	521	F	-1474.25	Japan
FL.20	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-1463.38	Germany
GS.1	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-1463.38	Germany
KP.3.1.3	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1453.47	Japan
B.1.1.348	S	NIID WH-1 F24381R24873	ATTTGAAACAAAGACACCTTCAC	23	34.78	24856	24834	R	-1450.82	Japan
B.1.1.412	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1441.38	South Korea
AY.43.8 (Delta)	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-1427.97	China
B.1.177.71	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-1427.97	China
B.1.1.445	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-1427.97	China
XBF.7.1	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-1427.97	China
B.1.1.149	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.491	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.619.1	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.177.59	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
AH.3	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.357	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.77	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.90	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.411	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
AJ.1	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
A.23	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.111	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.157	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
A.19	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.398	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.50	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.182	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
C.27	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
C.8	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
C.38	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.193	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.36.7	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.282	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.45	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.619	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.160.7	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
B.1.1.303	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1427.08	Japan
HK.24.1	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-1411.36	Japan
B.1.177.49	S	Young-S	ATCCAGCCTCTTATTATGTTAGAC	24	37.5	21876	21853	R	-1407.41	Singapore
AJ.1	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
AH.3	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
JN.1.10	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
HV.1.4	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
B	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
JN.1.51	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
GJ.1.2.4	S	WuhanCoV-spk1	TTGGCAAAATTCAAGACTCACTTT	24	33.33	24354	24377	F	-1403.3	Japan
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used