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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
JN.1.1.10	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.4	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
BA.2.86	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LE.1.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LE.1.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KB.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.16.3	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
MK.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KP.4.1.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KP.4.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LB.1.7.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.42.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LA.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LF.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KP.4	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KP.2.16	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.44	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LR.2	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
KR.4	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
LQ.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.4.8	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
EG.5.1.8	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
EG.5.1.14	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JD.1.1.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.32.1	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.34	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
JN.1.41	ORF1ab	NIID WH-1 F509R854	CTCGAACTGCACCTCATGG	19	57.89	492	510	F	-1981.59	Japan
GM.2	N	Chan-N	GCGTTCTTCGGAATGTCG	18	55.56	29210	29227	F	-1981.37	China
B.50	N	Chan-N	GCGTTCTTCGGAATGTCG	18	55.56	29210	29227	F	-1981.37	China
B.1.277	N	CN-CDC-N	GGGGAACTTCTCCTGCTAGAAT	22	50	28881	28902	F	-1936.12	China
GA.8	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
BA.1.23	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
AY.4.9 (Delta)	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
GE.1.2.1	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
FL.32.1	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
XDK.2	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
BN.1.3.12	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
CM.8.1.2	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
BF.7.4	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
B.1.637	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
B.50	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
XBB.1.38.1	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
BN.1.2.2	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
B.1.258.17	E	Won-E	CACACAATCGATGCGCAGTA	20	50	26365	26346	R	-1935.15	South Korea
B.50	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
B.1.267	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
B.1.537	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
B.1.548	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
B.19	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
AJ.1	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-1923.32	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used