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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
B.1.177.49	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.1.194	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.5.2.20	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BN.1.3	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.2.75	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.5.2	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
JN.1.30.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.4.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
KT.1.2	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
JN.1.7.7	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
JN.3.2	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.2.23	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.2 (Omicron)	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BN.1.3.12	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.2.9	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BN.1.3.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
unclassifiable	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
XBB.1.5.68	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.277	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.182	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.1.412	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
AY.105 (Delta)	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
GE.1.2.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
FL.39.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BA.2.75.10	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.1.348	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
XBB.1.5.97	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
XBB.1.11.1	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
B.1.595.3	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
FL.25	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
XBB.1.9	S	NIID WH-1 Seq F24383R24865	CAAAGACACCTTCACGAGG	19	52.63	24848	24830	R	-4566.9	Japan
BV.2	ORF1ab	Charité-ORF1b	GTGARATGGTCATGTGTGGCGG	22	54.55	15431	15452	F	-4521.82	Germany
XBC.1.6.1	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-4505.94	Germany
XBC.2	ORF1ab	Charité-ORF1b	CARATGTTAAASACACTATTAGCATA	26	23.08	15530	15505	R	-4505.94	Germany
XAY.2.1	S	NIID WH-1 F24381R24873	TCAAGACTCACTTTCTTCCAC	21	42.86	24364	24384	F	-4488.27	Japan
B.1.1.327	E	Niu-E	TTCTTGCTTTCGTGGTATTC	20	40	26303	26322	F	-4486.95	China
B.1.1.424	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.160.20	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.160.19	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.160.12	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.1.466	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.1.197	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.491	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
AY.79 (Delta)	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.57	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
AH.3	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.1.289	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.177.52	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.55	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
B.1.420	ORF1ab	Chan-ORF1ab	CGCATACAGTCTTRCAGGCT	20	50	16220	16239	F	-4484.57	China
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used