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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BN.1.3 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.5.1.22 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.2.75 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BF.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| CH.1.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BF.7.6 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.4.6.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.2.12.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.2.36 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BN.1.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BQ.1.1.8 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| CH.1.1.6 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.2.3 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.5.1.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| CH.1.1.15 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.4 (Omicron) | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.4.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.5.2.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| BA.1.1.3 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| B.1.1.194 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| B.1.1.303 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| B.1.36.22 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| B.1.398 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| B.1.258.4 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -4768.1 | Hong Kong |
| HK.16 | S | Chan-S | CAAGCTATAACGCAGCCTGTA | 21 | 47.62 | 22869 | 22849 | R | -4753.05 | China |
| JN.1.1.4 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| EK.4 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| BQ.1.22 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| BQ.1.1.72 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.232 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| BA.1.21 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| BA.1.1.16 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| AH.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.463 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.511 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.407 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.53 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.258.17 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.355 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| AY.5.1 (Delta) | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.289 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.121 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.356 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.6.8 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| AJ.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.98 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.384 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| AW.1 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.1.157 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| B.1.515 | N | SC2 | CTGCAGATTTGGATGATTTCTCC | 23 | 43.48 | 29463 | 29485 | F | -4751.81 | US |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |