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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| N.2 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.208 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.78 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.159 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.43.5 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XBC.1.6.4 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FP.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.2.2.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.2.2 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.443 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AP.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AK.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.98.1.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.1.13.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| HK.34.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.124.1.1 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.203 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| FY.8.1.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| GY.9 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.7.1 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.254 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.36.1 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.36.12 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.21 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.122.2 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AQ.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.1.1.7 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.209 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.236 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.2.33 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XQ | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.59 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.395 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| AY.120.2.1 (Delta) | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.416.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.532 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.408 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| XH | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BM.6 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.5.2.46 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| BA.5.7 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| CU.1 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| CA.2 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.83 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.40 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.161 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.58 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.177.75 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.411 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| B.1.1.217 | ORF1ab | Chan-ORF1ab | CGCATACAGTCTTRCAGGCT | 20 | 50 | 16220 | 16239 | F | -11502.5 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |