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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| CM.8 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.5.1.27 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BF.16 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.5.5.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.5.2.59 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BF.15 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BQ.1.15 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| CA.5 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| CP.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XBB.3.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| ED.3 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| CM.4 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BQ.1.4 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| CA.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BN.1.9 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BQ.1.28 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| DG.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| BA.5.2.56 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| KP.2.13 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| XDP.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HN.8 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| KL.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HK.24.1 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| GK.2.4 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| FL.1.5.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| HE.2 | ORF1ab | Pasteur-ORF1ab-1 | ATGAGCTTAGTCCTGTTG | 18 | 44.44 | 12690 | 12707 | F | -17837.6 | France |
| B.1.1.268 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| JN.1.4 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| JD.1.1.3 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| XBB.1.5.11 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| LL.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| AY.91.1 (Delta) | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| JB.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| FL.19 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| CH.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| XBB.1.5.54 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| B.1.160.9 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| B.31 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -17837 | South Korea |
| B.1.1.280 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| XBB.1.5.74 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| AE.5 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| XBB.1.5 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| MK.2 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| KP.2.3.4 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| XCT | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| HV.1.4 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| B.1.617.3 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| B.1.1.309 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| B.1.404 | ORF1ab | NIID WH-1 F509R854 | CAGAAGTTGTTATCGACATAGC | 22 | 40.91 | 837 | 816 | R | -17798.2 | Japan |
| JN.1.62 | N | NIH-TH_N | CGTTTGGTGGACCCTCAGAT | 20 | 55 | 28320 | 28339 | F | -17792.3 | Thailand |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |