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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| P.6 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.48 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.112 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.274 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.515 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.370 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.118 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.137 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.309 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.632 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.72 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.108 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.332 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.357 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BP.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.177.46 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.2.3.21 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| A.6 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.530 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.1.17.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.570 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.633 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| DD.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.136 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| DJ.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| XAE | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.467 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.484 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.476 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| A.19 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| A.18 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.404 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| DJ.1.3 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BQ.1.1.73 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| DW.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| XAK | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| XAA | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.25 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.5.9 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.7.4.3 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| ED.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| CQ.2 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.5.2.43 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.5.2.19 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.38.3 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.7.19.1 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BQ.1.1.43 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BA.5.2.12 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| BF.7.23 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| B.1.1.214 | ORF1ab | Charité-ORF1b | GTGARATGGTCATGTGTGGCGG | 22 | 54.55 | 15431 | 15452 | F | -20262.1 | Germany |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |