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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
D.3	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
AE.2	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.110.1	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.342	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.500	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.511	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.428.1	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.506	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.482	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.335	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.36.36	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.499.1	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.38	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.116	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.134	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.393	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.381	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
C.32	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
AE.8	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.516	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.1.526	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
B.1.279	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
AE.7	ORF1ab	Yip-ORF1ab	ATGCATTTGCATCAGAGGCT	20	45	1866	1885	F	-21402.5	China
GT.1	S	Young-S	ATCCAGCCTCTTATTATGTTAGAC	24	37.5	21876	21853	R	-21401.7	Singapore
XBB.2.11.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
EE.5	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BQ.1.1.52	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBF.9	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BN.1.4.5	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
DS.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BA.5.2.32	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BQ.1.1.58	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
B.1.631	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
B.1.627	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
B.1.1.519	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
B.1.1.514	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
GT.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBB.1.5.87	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XAP	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BE.1.4.3	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBB.2.6.3	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBB.1.47.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBF.2	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
CF.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
CM.11	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
DN.3.1	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
DJ.1.2	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
XBC.1.5	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
CM.6	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
BW.1.2	S	NIID WH-1 Seq F24383R24865	AAGACTCACTTTCTTCCACAG	21	42.86	24366	24386	F	-21400.8	Japan
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used