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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| JN.1.7.8 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.2.3.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| LB.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.3.2.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.3.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.1.1.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.2.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JN.1.7.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.1.1.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.2.15 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| KP.2.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.16.14 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EG.5.1.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FL.28 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FL.20.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.110 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| BA.2.86.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GN.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.8.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HB.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| EG.10.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.89 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HR.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HP.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GW.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GS.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XCF.3 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FL.34 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HY.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| JC.5 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.105 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.9.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GJ.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GK.1.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FY.4.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.103 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HC.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FE.1.1.4 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.94 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GK.7 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| GE.1.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HE.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.33 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FY.4.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.5.99 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HW.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| XBB.1.4.2 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| FE.1.1.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| HA.1 | N | Chan-N | TTGGATCTTTGTCATCCAATTTG | 23 | 34.78 | 29306 | 29284 | R | -21867.4 | China |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |