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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| XBB.1.5.33 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XDD.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XDD.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| BA.1.19 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HN.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JN.1.23 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XDS | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GJ.1.2.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HK.3.2.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HK.2.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GW.5.3.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.9 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HK.23.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JE.1.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.5.83 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.5.32 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HN.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.40 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GK.1.6.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GY.8 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XCW | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GA.2.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GK.3.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.2.3.21 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.36 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.5.108 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JS.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.5.104 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HH.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.1.6 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GA.4.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GW.5.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JE.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| EG.5.2.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| B.11 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| DV.7.1.5 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.16.23 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GN.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JH.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GJ.5 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GJ.1.2.2 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HN.4 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| HK.6 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| EG.10.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XBB.1.41.3 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| GW.5 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JG.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| XDC | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| FL.15.4 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| JR.1.1.1 | ORF1ab | NIID WH-1 F501R913 | CTTTACCAGCACGTGCTAGAAGG | 23 | 52.17 | 896 | 874 | R | -25657.5 | Japan |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |