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Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants.

Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used
BM.6	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
BA.5.2.46	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
BA.5.7	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
CU.1	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
CA.2	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.177.83	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.177.40	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.160.27	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.161	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.58	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.177.75	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.411	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.217	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.177.35	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
AY.17 (Delta)	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.41	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.527	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
C.18	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.500	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.147	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.1.487	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
B.1.177.68	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
AA.5	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
AS.2	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
AY.127.1 (Delta)	N	SC2	CTGCAGATTTGGATGATTTCTCC	23	43.48	29463	29485	F	-23117.2	US
CA.6	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
BM.6	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
BA.5.2.46	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
BA.5.7	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
CU.1	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
CA.2	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.177.40	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.160.27	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
AY.4.2.4 (Delta)	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.161	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.58	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.177.75	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.411	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.217	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.177.35	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
AY.17 (Delta)	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.41	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.527	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
C.18	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.500	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.147	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.487	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.177.68	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
B.1.1.121	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
AA.5	N	Won-N	GTTGCGACTACGTGATGAGG	20	55	28849	28830	R	-23088.1	South Korea
Lineage (WHO label)	Target Gene	Primer Name	Primer Sequence	Primer Length	Primer GC Content (%)	Start Position	End Position	Primer Direction	ΔG (cal)	Primer first used