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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| LP.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| LB.1.2.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| LB.1.2.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| LB.1.2.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| LB.1.2.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KP.3.3.3 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| KP.3.3.3 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| KP.3.3.3 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| KP.3.3.3 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| ML.2 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| ML.2 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| ML.2 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| ML.2 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KP.2.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| KP.2.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| KP.2.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| KP.2.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| KP.3.1.6 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| KP.3.1.6 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.2 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.2 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| JN.2 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.2 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JD.1.1.6 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JD.1.1.6 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| JD.1.1.6 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JD.1.1.6 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HK.3.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HK.3.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HK.3.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HK.3.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.9 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.1.9 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.1.9 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.9 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.7 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.1.7 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.1.7 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.7 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.43 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.1.43 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.1.43 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JN.1.43 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JN.1.21 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JN.1.21 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -14207.5 | Hong Kong |
| JN.1.21 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |