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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| AY.122 (Delta) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| AY.122 (Delta) | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| AY.122 (Delta) | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| B.1.1.7 (Alpha) | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| B.1.1.7 (Alpha) | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| B.1.1.7 (Alpha) | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| B.1.1.7 (Alpha) | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| B.1.1.7 (Alpha) | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -22650 | South Korea |
| B.1.1.7 (Alpha) | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| XCU | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -19758.2 | South Korea |
| XCU | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| XCU | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -17417.6 | South Korea |
| XCU | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| XCU | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| XCU | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| XCU | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| XCU | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| JN.6 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| JN.6 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JN.6 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| JN.6 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| JN.6 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| JN.6 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| JN.6 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| JN.6 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| HK.27.1 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -19758.2 | South Korea |
| HK.27.1 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| HK.27.1 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| HK.27.1 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| HK.27.1 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| HK.27.1 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| HK.27.1 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| HK.27.1 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| KB.2 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -19758.2 | South Korea |
| KB.2 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| KB.2 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| KB.2 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| KB.2 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -15016.3 | South Korea |
| KB.2 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| KB.2 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| KB.2 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -19777.6 | South Korea |
| JN.14 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -22753.7 | South Korea |
| JN.14 | ORF1ab | Won-ORF1ab | TGCATTAACATTGGCCGTGA | 20 | 45 | 15558 | 15539 | R | -21175.8 | South Korea |
| JN.14 | S | Won-S | CTACATGCACCAGCAACTGT | 20 | 50 | 23114 | 23133 | F | -22142.8 | South Korea |
| JN.14 | S | Won-S | CACCTGTGCCTGTTAAACCA | 20 | 50 | 23213 | 23194 | R | -21965.9 | South Korea |
| JN.14 | E | Won-E | TTCGGAAGAGACAGGTACGTT | 21 | 47.62 | 26259 | 26279 | F | -18253.7 | South Korea |
| JN.14 | E | Won-E | CACACAATCGATGCGCAGTA | 20 | 50 | 26365 | 26346 | R | -19261.4 | South Korea |
| JN.14 | N | Won-N | CAATGCTGCAATCGTGCTAC | 20 | 50 | 28732 | 28751 | F | -22388.1 | South Korea |
| JN.14 | N | Won-N | GTTGCGACTACGTGATGAGG | 20 | 55 | 28849 | 28830 | R | -23088.1 | South Korea |
| HK.32 | ORF1ab | Won-ORF1ab | CATGTGTGGCGGTTCACTAT | 20 | 50 | 15441 | 15460 | F | -19758.2 | South Korea |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |