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| Prediction results retrieved after performance evaluation of WHO approved 34 primers to estimate their binding affinities for 3892 SARS-CoV-2 variants. |
| Lineage (WHO label)   | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |
|---|---|---|---|---|---|---|---|---|---|---|
| BA.2.78 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| BA.2.78 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BA.2.78 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| BA.2.78 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| BA.2.43 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| BA.2.43 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| BA.2.43 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| BA.2.43 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HR.1.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HR.1.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HR.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -17559.5 | Hong Kong |
| HR.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HK.7 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HK.7 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HK.7 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HK.7 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HS.1.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HS.1.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HS.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HS.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HH.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HH.5 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HH.5 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HH.5 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HU.1.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HU.1.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| HU.1.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| HU.1.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| HV.1.5 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| HV.1.5 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| GA.7 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| FL.23 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| FL.23 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| FL.23 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| FL.23 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| XBB.2.3.13 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| XBB.2.3.13 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| XBB.2.3.13 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| XBB.2.3.13 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| B.1.160 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| B.1.160 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| B.1.160 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| B.1.160 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| JV.1 | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| JV.1 | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| JV.1 | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| JV.1 | N | HKU-N | CGAAGGTGTGACTTCCATG | 19 | 52.63 | 29254 | 29236 | R | -20936.4 | Hong Kong |
| XCY | ORF1ab | HKU-ORF1b | TGGGGYTTTACRGGTAACCT | 20 | 45 | 18778 | 18797 | F | -7043.36 | Hong Kong |
| XCY | ORF1ab | HKU-ORF1b | AACRCGCTTAACAAAGCACTC | 21 | 42.86 | 18909 | 18889 | R | -17655.9 | Hong Kong |
| XCY | N | HKU-N | TAATCAGACAAGGAACTGATTA | 22 | 31.82 | 29145 | 29166 | F | -18800.2 | Hong Kong |
| Lineage (WHO label) | Target Gene | Primer Name | Primer Sequence | Primer Length | Primer GC Content (%) | Start Position | End Position | Primer Direction | ΔG (cal) | Primer first used |